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adrb2 gene  (OriGene)


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    OriGene adrb2 gene
    Functional cAMP responses to a panel of β-AR agonists in the C6 rat glioma cell line with (A) native expression, (B) Adrb1 (β 1 -AR) knockout, or (C) <t>Adrb2</t> (β 2 -AR) knockout. Representative curves are displayed, each with four technical replicates, showing mean ± SEM cAMP HTRF responses normalized to the full agonist isoprenaline. Each agonist curve was converted to a Δlog(E max /EC 50 ) agonist fingerprint using isoprenaline as the reference molecule. Radar plots below the concentration–response curves demonstrate an agonist fingerprint of the C6 cell line non-overlapping with human β 1 -AR or β 2 -AR receptors, suggesting possible species differences and/or dual-receptor expression. (D) Overlay of agonist fingerprints across species for single-receptor systems from either rat (C6 knockout) or human (CHO-K1 recombinant), with an intermediate profile from the C6 co-expressing cell line. (E) Rat primary astrocytes display agonist responses and an agonist fingerprint with hybrid features (F) , indicating endogenous co-expression of β 1 -AR and β 2 -AR.
    Adrb2 Gene, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nm+000024/pmc10471193-46-19-21?v=OriGene
    Average 91 stars, based on 1 article reviews
    adrb2 gene - by Bioz Stars, 2026-06
    91/100 stars

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    1) Product Images from "Fingerprinting heterocellular β-adrenoceptor functional expression in the brain using agonist activity profiles"

    Article Title: Fingerprinting heterocellular β-adrenoceptor functional expression in the brain using agonist activity profiles

    Journal: Frontiers in Molecular Biosciences

    doi: 10.3389/fmolb.2023.1214102

    Functional cAMP responses to a panel of β-AR agonists in the C6 rat glioma cell line with (A) native expression, (B) Adrb1 (β 1 -AR) knockout, or (C) Adrb2 (β 2 -AR) knockout. Representative curves are displayed, each with four technical replicates, showing mean ± SEM cAMP HTRF responses normalized to the full agonist isoprenaline. Each agonist curve was converted to a Δlog(E max /EC 50 ) agonist fingerprint using isoprenaline as the reference molecule. Radar plots below the concentration–response curves demonstrate an agonist fingerprint of the C6 cell line non-overlapping with human β 1 -AR or β 2 -AR receptors, suggesting possible species differences and/or dual-receptor expression. (D) Overlay of agonist fingerprints across species for single-receptor systems from either rat (C6 knockout) or human (CHO-K1 recombinant), with an intermediate profile from the C6 co-expressing cell line. (E) Rat primary astrocytes display agonist responses and an agonist fingerprint with hybrid features (F) , indicating endogenous co-expression of β 1 -AR and β 2 -AR.
    Figure Legend Snippet: Functional cAMP responses to a panel of β-AR agonists in the C6 rat glioma cell line with (A) native expression, (B) Adrb1 (β 1 -AR) knockout, or (C) Adrb2 (β 2 -AR) knockout. Representative curves are displayed, each with four technical replicates, showing mean ± SEM cAMP HTRF responses normalized to the full agonist isoprenaline. Each agonist curve was converted to a Δlog(E max /EC 50 ) agonist fingerprint using isoprenaline as the reference molecule. Radar plots below the concentration–response curves demonstrate an agonist fingerprint of the C6 cell line non-overlapping with human β 1 -AR or β 2 -AR receptors, suggesting possible species differences and/or dual-receptor expression. (D) Overlay of agonist fingerprints across species for single-receptor systems from either rat (C6 knockout) or human (CHO-K1 recombinant), with an intermediate profile from the C6 co-expressing cell line. (E) Rat primary astrocytes display agonist responses and an agonist fingerprint with hybrid features (F) , indicating endogenous co-expression of β 1 -AR and β 2 -AR.

    Techniques Used: Functional Assay, Expressing, Knock-Out, Concentration Assay, Recombinant

    Functional cAMP responses to a panel of β-AR agonists in the human THP-1 cell line with (A) native expression, (B) Adrb1 (β 1 -AR) knockout, or (C) Adrb2 (β 2 -AR) knockout. Representative curves are displayed, each with four technical replicates, showing mean ± SEM cAMP HTRF responses normalized to the full agonist isoprenaline. Each agonist curve was converted to a Δlog(E max /EC 50 ) agonist fingerprint using isoprenaline as the reference molecule. Radar plots below the concentration–response curves reveal an agonist fingerprint of the THP-1 cell line, suggestive of predominantly β 1 -AR function. (D) Overlay of agonist fingerprints across human cell lines with either endogenous or exogenous receptor expression shows good agreement for single-receptor systems. (E) Gene knockout of individual receptors in the THP-1 cell line confirms that the functional cAMP response in parental THP-1 cells is driven predominantly by β 1 -AR receptors; the left subpanel shows cAMP concentration after normalization of the HTRF response.
    Figure Legend Snippet: Functional cAMP responses to a panel of β-AR agonists in the human THP-1 cell line with (A) native expression, (B) Adrb1 (β 1 -AR) knockout, or (C) Adrb2 (β 2 -AR) knockout. Representative curves are displayed, each with four technical replicates, showing mean ± SEM cAMP HTRF responses normalized to the full agonist isoprenaline. Each agonist curve was converted to a Δlog(E max /EC 50 ) agonist fingerprint using isoprenaline as the reference molecule. Radar plots below the concentration–response curves reveal an agonist fingerprint of the THP-1 cell line, suggestive of predominantly β 1 -AR function. (D) Overlay of agonist fingerprints across human cell lines with either endogenous or exogenous receptor expression shows good agreement for single-receptor systems. (E) Gene knockout of individual receptors in the THP-1 cell line confirms that the functional cAMP response in parental THP-1 cells is driven predominantly by β 1 -AR receptors; the left subpanel shows cAMP concentration after normalization of the HTRF response.

    Techniques Used: Functional Assay, Expressing, Knock-Out, Concentration Assay, Gene Knockout



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    Functional cAMP responses to a panel of β-AR agonists in the C6 rat glioma cell line with (A) native expression, (B) Adrb1 (β 1 -AR) knockout, or (C) <t>Adrb2</t> (β 2 -AR) knockout. Representative curves are displayed, each with four technical replicates, showing mean ± SEM cAMP HTRF responses normalized to the full agonist isoprenaline. Each agonist curve was converted to a Δlog(E max /EC 50 ) agonist fingerprint using isoprenaline as the reference molecule. Radar plots below the concentration–response curves demonstrate an agonist fingerprint of the C6 cell line non-overlapping with human β 1 -AR or β 2 -AR receptors, suggesting possible species differences and/or dual-receptor expression. (D) Overlay of agonist fingerprints across species for single-receptor systems from either rat (C6 knockout) or human (CHO-K1 recombinant), with an intermediate profile from the C6 co-expressing cell line. (E) Rat primary astrocytes display agonist responses and an agonist fingerprint with hybrid features (F) , indicating endogenous co-expression of β 1 -AR and β 2 -AR.
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    Functional cAMP responses to a panel of β-AR agonists in the C6 rat glioma cell line with (A) native expression, (B) Adrb1 (β 1 -AR) knockout, or (C) <t>Adrb2</t> (β 2 -AR) knockout. Representative curves are displayed, each with four technical replicates, showing mean ± SEM cAMP HTRF responses normalized to the full agonist isoprenaline. Each agonist curve was converted to a Δlog(E max /EC 50 ) agonist fingerprint using isoprenaline as the reference molecule. Radar plots below the concentration–response curves demonstrate an agonist fingerprint of the C6 cell line non-overlapping with human β 1 -AR or β 2 -AR receptors, suggesting possible species differences and/or dual-receptor expression. (D) Overlay of agonist fingerprints across species for single-receptor systems from either rat (C6 knockout) or human (CHO-K1 recombinant), with an intermediate profile from the C6 co-expressing cell line. (E) Rat primary astrocytes display agonist responses and an agonist fingerprint with hybrid features (F) , indicating endogenous co-expression of β 1 -AR and β 2 -AR.
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    Functional cAMP responses to a panel of β-AR agonists in the C6 rat glioma cell line with (A) native expression, (B) Adrb1 (β 1 -AR) knockout, or (C) <t>Adrb2</t> (β 2 -AR) knockout. Representative curves are displayed, each with four technical replicates, showing mean ± SEM cAMP HTRF responses normalized to the full agonist isoprenaline. Each agonist curve was converted to a Δlog(E max /EC 50 ) agonist fingerprint using isoprenaline as the reference molecule. Radar plots below the concentration–response curves demonstrate an agonist fingerprint of the C6 cell line non-overlapping with human β 1 -AR or β 2 -AR receptors, suggesting possible species differences and/or dual-receptor expression. (D) Overlay of agonist fingerprints across species for single-receptor systems from either rat (C6 knockout) or human (CHO-K1 recombinant), with an intermediate profile from the C6 co-expressing cell line. (E) Rat primary astrocytes display agonist responses and an agonist fingerprint with hybrid features (F) , indicating endogenous co-expression of β 1 -AR and β 2 -AR.
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    Image Search Results


    Functional cAMP responses to a panel of β-AR agonists in the C6 rat glioma cell line with (A) native expression, (B) Adrb1 (β 1 -AR) knockout, or (C) Adrb2 (β 2 -AR) knockout. Representative curves are displayed, each with four technical replicates, showing mean ± SEM cAMP HTRF responses normalized to the full agonist isoprenaline. Each agonist curve was converted to a Δlog(E max /EC 50 ) agonist fingerprint using isoprenaline as the reference molecule. Radar plots below the concentration–response curves demonstrate an agonist fingerprint of the C6 cell line non-overlapping with human β 1 -AR or β 2 -AR receptors, suggesting possible species differences and/or dual-receptor expression. (D) Overlay of agonist fingerprints across species for single-receptor systems from either rat (C6 knockout) or human (CHO-K1 recombinant), with an intermediate profile from the C6 co-expressing cell line. (E) Rat primary astrocytes display agonist responses and an agonist fingerprint with hybrid features (F) , indicating endogenous co-expression of β 1 -AR and β 2 -AR.

    Journal: Frontiers in Molecular Biosciences

    Article Title: Fingerprinting heterocellular β-adrenoceptor functional expression in the brain using agonist activity profiles

    doi: 10.3389/fmolb.2023.1214102

    Figure Lengend Snippet: Functional cAMP responses to a panel of β-AR agonists in the C6 rat glioma cell line with (A) native expression, (B) Adrb1 (β 1 -AR) knockout, or (C) Adrb2 (β 2 -AR) knockout. Representative curves are displayed, each with four technical replicates, showing mean ± SEM cAMP HTRF responses normalized to the full agonist isoprenaline. Each agonist curve was converted to a Δlog(E max /EC 50 ) agonist fingerprint using isoprenaline as the reference molecule. Radar plots below the concentration–response curves demonstrate an agonist fingerprint of the C6 cell line non-overlapping with human β 1 -AR or β 2 -AR receptors, suggesting possible species differences and/or dual-receptor expression. (D) Overlay of agonist fingerprints across species for single-receptor systems from either rat (C6 knockout) or human (CHO-K1 recombinant), with an intermediate profile from the C6 co-expressing cell line. (E) Rat primary astrocytes display agonist responses and an agonist fingerprint with hybrid features (F) , indicating endogenous co-expression of β 1 -AR and β 2 -AR.

    Article Snippet: ReNcell VM cells expressing β 2 -AR were generated by infecting ReNcell VM cells with a lentivirus containing the ADRB2 gene (OriGene RC204499L3V) at an MOI of 12.5, and 48 h post-infection, cells were grown in media containing 0.25 μg/mL puromycin.

    Techniques: Functional Assay, Expressing, Knock-Out, Concentration Assay, Recombinant

    Functional cAMP responses to a panel of β-AR agonists in the human THP-1 cell line with (A) native expression, (B) Adrb1 (β 1 -AR) knockout, or (C) Adrb2 (β 2 -AR) knockout. Representative curves are displayed, each with four technical replicates, showing mean ± SEM cAMP HTRF responses normalized to the full agonist isoprenaline. Each agonist curve was converted to a Δlog(E max /EC 50 ) agonist fingerprint using isoprenaline as the reference molecule. Radar plots below the concentration–response curves reveal an agonist fingerprint of the THP-1 cell line, suggestive of predominantly β 1 -AR function. (D) Overlay of agonist fingerprints across human cell lines with either endogenous or exogenous receptor expression shows good agreement for single-receptor systems. (E) Gene knockout of individual receptors in the THP-1 cell line confirms that the functional cAMP response in parental THP-1 cells is driven predominantly by β 1 -AR receptors; the left subpanel shows cAMP concentration after normalization of the HTRF response.

    Journal: Frontiers in Molecular Biosciences

    Article Title: Fingerprinting heterocellular β-adrenoceptor functional expression in the brain using agonist activity profiles

    doi: 10.3389/fmolb.2023.1214102

    Figure Lengend Snippet: Functional cAMP responses to a panel of β-AR agonists in the human THP-1 cell line with (A) native expression, (B) Adrb1 (β 1 -AR) knockout, or (C) Adrb2 (β 2 -AR) knockout. Representative curves are displayed, each with four technical replicates, showing mean ± SEM cAMP HTRF responses normalized to the full agonist isoprenaline. Each agonist curve was converted to a Δlog(E max /EC 50 ) agonist fingerprint using isoprenaline as the reference molecule. Radar plots below the concentration–response curves reveal an agonist fingerprint of the THP-1 cell line, suggestive of predominantly β 1 -AR function. (D) Overlay of agonist fingerprints across human cell lines with either endogenous or exogenous receptor expression shows good agreement for single-receptor systems. (E) Gene knockout of individual receptors in the THP-1 cell line confirms that the functional cAMP response in parental THP-1 cells is driven predominantly by β 1 -AR receptors; the left subpanel shows cAMP concentration after normalization of the HTRF response.

    Article Snippet: ReNcell VM cells expressing β 2 -AR were generated by infecting ReNcell VM cells with a lentivirus containing the ADRB2 gene (OriGene RC204499L3V) at an MOI of 12.5, and 48 h post-infection, cells were grown in media containing 0.25 μg/mL puromycin.

    Techniques: Functional Assay, Expressing, Knock-Out, Concentration Assay, Gene Knockout